Anti-MKP1 Rabbit Polyclonal Antibody for WB, ELISA - P28563

Core Product Specifications and Parameters

Biological Background: MKP1 Function and Localization

• MKP1 (DUSP1) is a dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both Thr-183 and Tyr-185, thereby negatively regulating MAP kinase signaling during the meiotic cell cycle.
• The protein localizes predominantly to the nucleus, where it modulates nuclear ERK2 activity.
• It belongs to the dual specificity protein phosphatase family, capable of removing phosphate groups from phosphotyrosine and phosphothreonine residues.
• Also known as 3CH134, ERP, and Ptpn16, it is encoded by the Dusp1 gene in mouse.
• Keywords from UniProt indicate it is a phosphoprotein and undergoes ubiquitin conjugation, suggesting regulation by post-translational modifications.
• The predicted molecular weight is approximately 39 kDa, consistent with the provided protein weight.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein spanning amino acids 1-172 of mouse MKP1. It is suitable for Western blot and ELISA applications.
• For Western blot, the expected band size is around 39 kDa; we recommend optimizing loading amounts and antibody dilutions for your specific sample type.
• The antibody shows cross-reactivity with human, mouse, and rat MKP1, but in-species validation is advised to confirm performance.
• For ELISA, the antibody may be used for direct or indirect detection; consider pairing with an appropriate secondary antibody and optimizing coating conditions.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to qualify each new lot for your intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material sourcing partner, connecting diagnostic developers with essential reagents from concept to clinic. This MKP1 antibody supports MAP kinase signaling research and assay development. We offer validated antibodies, bulk ancillary reagents, and sourcing of rare targets to streamline your procurement process.