Anti-MKP1 Rabbit Polyclonal Antibody for WB, ELISA - P28562

Core Product Specifications and Parameters

Biological Background: MKP1 Function and Localization

• Dual specificity protein phosphatase 1 (DUSP1, also known as MKP-1, hVH1, CL100, PTPN10) is a member of the dual specificity phosphatase family that dephosphorylates both phosphotyrosine and phosphoserine/threonine residues.
• It specifically dephosphorylates MAP kinase MAPK1/ERK2 on 'Thr-183' and 'Tyr-185', thereby regulating its activity during the meiotic cell cycle.
• DUSP1 is localized primarily in the nucleus.
• It is expressed at high levels in the lung, liver, placenta, and pancreas, with moderate levels in heart and skeletal muscle, and lower levels in brain and kidney.
• The protein undergoes post-translational modifications, including phosphorylation and ubiquitin conjugation (Ubl conjugation), which may regulate its stability and activity.
• DUSP1 is involved in stress response and cell cycle regulation, as indicated by its UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 267-367 of human MKP1. This region resides within the catalytic domain, which may influence antibody specificity. Verify in your experimental context.
• For Western blot (WB), the predicted molecular weight of MKP1 is 39 kDa. Use this as a guide and validate with appropriate molecular weight markers and cell/tissue lysates.
• This antibody is recommended for WB and ELISA. For other applications, such as immunohistochemistry or immunoprecipitation, empirical optimization is advised.
• Consider cross-reactivity with mouse MKP1; if working with mouse samples, confirm reactivity under your specific conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs and bulk ancillary reagents for MAP kinase signaling and stress response studies. Our anti-MKP1 polyclonal antibody supports WB and ELISA applications, and we offer customization and sourcing services for rare target raw materials. Partner with CamelBio to accelerate your assay development from concept to clinic with reliable, comprehensive supply solutions.