Anti-MKNK1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BUB5

Core Product Specifications and Parameters

Biological Background: MKNK1 Function and Localization

• MKNK1, also known as MAP kinase-interacting serine/threonine-protein kinase 1 or Mnk1, is a serine/threonine-protein kinase encoded by the MKNK1 gene in humans.
• It participates in cellular responses to environmental stress and cytokines, influencing downstream signaling cascades.
• A key function of MKNK1 is the phosphorylation of eukaryotic translation initiation factor 4E (EIF4E), which enhances the affinity of EIF4E for the 7-methylguanosine cap of mRNA, thereby regulating translation initiation.
• The kinase is classified under the CAMK family and requires magnesium and ATP for its activity, with a molecular weight of approximately 51 kDa (UniProt: 51342 Da).
• MKNK1 is ubiquitously expressed across various human tissues, reflecting its broad regulatory role.
• Subcellularly, the protein shuttles between the cytoplasm and the nucleus, consistent with its involvement in translation and possible nuclear functions.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 374-465) lies within the C-terminal region of MKNK1, which is less conserved among kinases and may confer specificity; consider using this information for blocking peptide design or epitope mapping.
• For Western blot (WB), a band around 51 kDa is expected; ensure proper positive controls using human, mouse, or rat cell lysates known to express MKNK1.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the staining pattern in relevant tissue sections or cells, and confirm localization to both cytoplasm and nucleus where expected.
• ELISA performance can be assessed using recombinant MKNK1 protein or peptide; optimize coating and detection conditions based on the polyclonal nature of the antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for IVD manufacturers and researchers, providing this anti-MKNK1 antibody alongside a broad portfolio of raw materials targeting kinases and translation regulation pathways. From validated polyclonal and monoclonal antibodies to recombinant proteins and ancillary reagents, we support your assay development with reliable supply chains and technical consultation. For customized requests, including alternative formats or bulk orders, our team is ready to bridge your research to clinical application.