Anti-MKLN1 Rabbit Polyclonal Antibody for WB and ELISA - Q9UL63

Core Product Specifications and Parameters

Biological Background: MKLN1 Function and Localization

• MKLN1 encodes Muskelin, a component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Related references: PMID:29911972
• It acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1. Related references: PMID:18710924
• Muskelin is required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent degradation (By similarity).
• The protein localizes to multiple subcellular compartments, including the cytoplasm, cytosol, nucleus, nucleoplasm, cell projections (ruffles), cell cortex, and postsynaptic sites.
• It contains Kelch repeat domains and undergoes acetylation, with alternative splicing producing multiple isoforms.
• The protein is part of the reference proteome and has been identified in proteomics screens.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 436-735, targeting the C-terminal region; consider this when designing epitope competition or truncation experiments.
• For Western blot, the predicted molecular weight is ~85 kDa, but alternative splicing may yield additional bands; validate in your target sample system.
• Due to cross-reactivity with mouse, this antibody can be used for comparative studies; verify species-specific expression patterns.
• For ELISA, include appropriate positive and negative controls to confirm specificity, especially when analyzing native protein conformations.
• Endogenous expression levels of MKLN1 vary across cell types; optimization of sample loading and antibody dilution is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including this anti-MKLN1 antibody for ubiquitin-proteasome and cell adhesion studies. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your development pipeline. For rare or hard-to-source targets, our team facilitates customized raw-material procurement to support your research from concept to clinic.