Anti-MKL2 (MRTFB) Rabbit Polyclonal Antibody for WB & ELISA - Q9ULH7

Core Product Specifications and Parameters

Biological Background: MKL2 Function and Localization

• MKL2, also known as myocardin-related transcription factor B (MRTFB) or megakaryoblastic leukemia 2, is a member of the myocardin family of transcriptional coactivators.
• It functions as a transcriptional coactivator of serum response factor (SRF), playing a crucial role in the regulation of immediate-early genes and muscle-specific gene expression.
• MKL2 is required for skeletal myogenic differentiation, highlighting its importance in developmental processes.
• The protein is primarily localized to the nucleus, where it exerts its transcriptional regulatory functions.
• Post-translational modifications include phosphorylation, glycosylation, isopeptide bond formation, and ubiquitin-like conjugation, which may modulate its activity and stability.
• Alternative splicing generates multiple isoforms of MKL2, potentially contributing to functional diversity.
• Keywords: chromosomal rearrangement (associated with megakaryoblastic leukemia), coiled coil domain, and repeat regions, suggesting structural motifs that mediate protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 170–300 of human MKL2, a region that may include functional domains; consider the accessibility of this epitope in your specific assay context.
• For Western blotting, MKL2 is predicted at 118 kDa; use an appropriate molecular weight marker and consider that post-translational modifications may slightly alter migration.
• In ELISA applications, the recombinant immunogen can serve as a positive control; optimize coating buffer and antibody dilution for best signal-to-noise ratio.
• As a polyclonal antibody, always validate a new lot by testing against your target sample system to confirm consistent reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutions a streamlined supply chain for IVD raw materials. For targets like MKL2, we provide ready-to-use antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Our sourcing network also covers rare targets—contact us to discuss your specific requirements.