Anti-MKL1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q969V6

Core Product Specifications and Parameters

Biological Background: MKL1 Function and Localization

• MKL1 (Myocardin-related transcription factor A), also known as MRTF-A, MAL, BSAC, is a member of the myocardin family of transcriptional coactivators.
• It associates with serum response factor (SRF) to drive expression of cytoskeletal genes, playing key roles in development, morphogenesis, and cell migration. Related references: PMID:26224645
• MKL1 activity is tightly regulated by the Rho GTPase signaling pathway through changes in cellular G-actin and F-actin levels; it binds G-actin via its RPEL repeats, and nuclear F-actin also modulates activity.
• Predominantly localizes to the cytoplasm, but translocates to the nucleus upon actin polymerization changes, enabling SRF-mediated transcription.
• Ubiquitously expressed across tissues including lung, placenta, intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. Related references: PMID:11344311; PMID:26224645
• Contains a coiled-coil domain, RPEL actin-binding repeats, and is regulated by phosphorylation; classified as a proto-oncogene, with chromosomal rearrangements linked to megakaryoblastic leukemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-280) of MKL1, which includes the RPEL motifs critical for G-actin binding; thus, antibody binding may be influenced by actin interactions in certain contexts.
• For Western blot (WB), the predicted molecular weight is ~99 kDa; validate detection in relevant human, mouse, or rat cell lines.
• In immunofluorescence (IF/ICC), monitor MKL1 nuclear translocation as a readout for SRF pathway activation; serum stimulation or Rho activation can be used as positive controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and validate with appropriate tissue controls due to ubiquitous expression.
• ELISA can be used for quantitative detection; consider pairing with a capture antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide validated IVD raw materials, including this anti-MKL1 polyclonal antibody targeting transcription and cytoskeletal biology. Our portfolio includes optimized antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic.