Anti-MKL1 Polyclonal Antibody for WB, ELISA - Q969V6

Core Product Specifications and Parameters

Biological Background: MKL1 Function and Localization

• MKL1 (Myocardin-related transcription factor A, also known as MRTF-A, MAL, or BSAC) is encoded by the MRTFA gene and belongs to the myocardin family of transcriptional coactivators.
• It associates with serum response factor (SRF) to regulate expression of genes controlling cytoskeletal dynamics during development, morphogenesis, and cell migration. Related references: PMID:26224645
• Activity is modulated by Rho GTPase signaling and cellular actin dynamics; MKL1 binds G-actin via its RPEL repeats, and nuclear F-actin levels further regulate the MRTFA-SRF complex.
• MKL1 localizes to both the cytoplasm and nucleus, undergoing nucleocytoplasmic shuttling in response to stimuli.
• It is ubiquitously expressed across tissues, including lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain, as well as peripheral blood mononuclear cells. Related references: PMID:11344311, PMID:26224645
• The protein is subject to phosphorylation (phosphoprotein keyword) and has been identified as a proto-oncogene, with chromosomal rearrangements reported in some leukemias.
• With a calculated molecular weight of approximately 99 kDa (SWISS Q969V6), MKL1 contains RPEL repeats and a coiled-coil domain, features typical of actin-binding transcriptional regulators.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody covers the N-terminal region (amino acids 1–280) of human MKL1, which includes the RPEL repeats; this may favor recognition of the actin-free, active form.
• For Western blot (WB), the expected band is around 99 kDa; consider using whole-cell lysates from human, mouse, or rat cell lines, and include appropriate loading controls.
• For ELISA applications, this antibody may be used as a capture or detection reagent; pairing with a specific MKL1 monoclonal antibody should be evaluated in the relevant sample system.
• Cross-reactivity with mouse and rat has been confirmed by supplier, but antibody performance in other applications (IHC, IF, etc.) has not been validated; users should perform their own optimization.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines procurement from concept to clinic, supplying validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For transcriptional regulation and cytoskeletal signaling research, we offer this anti-MKL1 polyclonal antibody along with complementary reagents to support your assay development. Contact us to source rare-target raw materials or customize antibody panels for your diagnostic platform.