Anti-Mki67 Rabbit Polyclonal Antibody for IF-P, IHC-P, ELISA - E9PVX6

Core Product Specifications and Parameters

Biological Background: Mki67 Function and Localization

• Mki67 encodes the proliferation marker protein Ki-67, a large nuclear protein (~350 kDa) that associates with mitotic chromosomes.
• During early mitosis, Ki-67 forms extended brush-like structures on chromosome surfaces, acting as a chromosome repellent that prevents chromosome collapse into a single mass, thereby enabling independent chromosome motility. Related references: PMID:27362226
• In late mitosis, Ki-67 switches to a chromosome attractant, promoting chromosome clustering and nuclear envelope reformation, likely via dephosphorylation and RNA-dependent liquid-liquid phase separation.
• Ki-67 does not contribute to the internal architecture of mitotic chromosomes but may influence chromatin organization indirectly. Related references: PMID:26949251
• Subcellular localization includes the nucleolus in interphase and relocalization to the chromosome periphery during mitosis.
• It is preferentially expressed in proliferating cells, making it a widely used marker for cell proliferation in research and diagnostics.
• The protein contains multiple repeats, is subject to acetylation, phosphorylation, and ubiquitination, and has ATP- and DNA-binding activities.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1816–2163 of mouse Mki67, a region within the C-terminal repeat domain; consider verifying reactivity with your specific sample type and application conditions.
• This antibody is suitable for paraffin-embedded immunohistochemistry (IHC-P), immunofluorescence on paraffin sections (IF-P), and ELISA. For each assay, optimization of antibody dilution, antigen retrieval, and detection system may be needed.
• Cross-reactivity is reported for mouse and rat; assess potential reactivity with other species if required.
• For proliferation studies, use continually dividing tissues (e.g., intestinal crypts, lymphoid organs) or tumor samples as positive controls to confirm expected staining patterns.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides a streamlined procurement channel for anti-Mki67 antibodies and ancillary reagents tailored for cell proliferation and oncology diagnostics. Beyond this rabbit polyclonal antibody, we offer validated monoclonal antibodies, antibody pairs, bulk reagents, and custom sourcing for rare targets to support your diagnostic development from concept to clinic.