Anti-Mitofusin 2 (MFN2) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95140

Core Product Specifications and Parameters

Biological Background: Mitofusin 2 Function and Localization

• Mitofusin-2 (MFN2) is a mitochondrial outer membrane transmembrane GTPase essential for mediating mitochondrial clustering and fusion. Related references: PMID:11181170, PMID:11950885, PMID:19889647
• Overexpression of MFN2 induces the formation of mitochondrial networks, highlighting its key role in mitochondrial dynamics. Related references: PMID:28114303
• MFN2 is required for PRKN recruitment to dysfunctional mitochondria and the selective autophagic clearance of damaged mitochondria (mitophagy). Related references: PMID:23620051
• The protein plays a central role in mitochondrial metabolism, apoptosis, and vascular smooth muscle cell proliferation, and also participates in the regulation of the unfolded protein response (UPR) during ER stress.
• Ubiquitously expressed at low levels, with elevated expression in heart and kidney, MFN2 localizes to the mitochondrial outer membrane.
• Pathogenic variants in MFN2 are associated with Charcot-Marie-Tooth disease type 2A and other neurodegenerative disorders.

Experimental Guidance and Technical Tips

• For Western blot (WB), the antibody detects a band at approximately 86 kDa corresponding to endogenous Mitofusin 2. Optimize loading and transfer conditions for this relatively large protein.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect a mitochondrial staining pattern; use mitochondrial markers for co-localization to confirm specificity.
• This polyclonal antibody, raised against a peptide within amino acids 70-260, is suitable for ELISA applications; consider using the immunizing peptide as a positive control.
• Cross-reactivity is cited for human, mouse, and rat samples; validate performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials and technical services. This anti-Mitofusin 2 polyclonal antibody supports mitochondrial biology and metabolism research, and we also offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For rare or custom targets, our sourcing team delivers tailored solutions to accelerate your project from concept to clinic.