Anti-Mitofusin-1/MFN1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8IWA4

Core Product Specifications and Parameters

Biological Background: Mitofusin-1 Function and Localization

• Mitofusin-1 (MFN1) is a dynamin-like GTPase located on the mitochondrial outer membrane, also known as Fzo homolog.
• It plays a central role in mediating mitochondrial clustering and fusion, which is essential for maintaining mitochondrial morphology and network dynamics. Related references: PMID:12475957 PMID:12759376 PMID:27920125
• MFN1-mediated membrane fusion requires its GTPase activity and likely involves major rearrangements of its coiled coil domains. Related references: PMID:27920125 PMID:28114303
• The protein is ubiquitously expressed, with relatively higher levels detected in kidney and heart tissues. Related references: PMID:11950885 PMID:12759376
• MFN1 undergoes alternative splicing to produce isoforms; isoform 2 may be overexpressed in certain tumors, such as lung cancers. Related references: PMID:11751411
• Keywords from UniProt annotation highlight its structural features: coiled coil domains, GTP-binding, nucleotide-binding, transmembrane helices, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (amino acids 642–741) of human MFN1, supporting detection of the full-length protein.
• For Western blot (WB) applications, expect a band near 84 kDa, consistent with the observed protein weight and the theoretical molecular mass of ~84.16 kDa.
• In immunohistochemistry (IHC-P), consider using human kidney or heart tissue sections as positive controls, where MFN1 expression is known to be high.
• For ELISA, validate antibody binding to the target in the relevant sample matrix, and titrate the antibody to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this high-quality recombinant rabbit monoclonal antibody against Mitofusin-1 as part of a comprehensive portfolio of IVD raw materials. We support diagnostic manufacturers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and dedicated sourcing for rare or custom targets—helping you advance mitochondrial research and diagnostic applications from concept to clinic.