Anti-Mitofusin-1/MFN1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IWA4

Core Product Specifications and Parameters

Biological Background: Mitofusin-1 Function and Localization

• Mitofusin-1 (MFN1), also referred to as Fzo homolog or transmembrane GTPase MFN1, is encoded by the MFN1 gene (Gene ID: 55669) and is a key mediator of mitochondrial outer membrane fusion.
• MFN1 functions as a mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion, a process essential for maintaining mitochondrial network morphology (PubMed:12475957, PubMed:12759376, PubMed:27920125).
  Related references:
  PMID:12475957
  PMID:12759376
  PMID:27920125
• Overexpression of MFN1 induces the formation of extensive mitochondrial networks in vitro, highlighting its role in promoting mitochondrial connectivity (PubMed:12759376).
  Related references:
  PMID:12759376
• MFN1 possesses low intrinsic GTPase activity, which is required for membrane clustering, and its function may involve major rearrangements of coiled-coil domains (PubMed:27920125, PubMed:28114303).
  Related references:
  PMID:27920125
  PMID:28114303
• The protein localizes primarily to the mitochondrial outer membrane but can also be found in the cytoplasm.
• MFN1 is ubiquitously expressed, with slightly higher levels in kidney and heart; isoform 2 may be overexpressed in certain tumors such as lung cancers (PubMed:11751411, PubMed:11950885, PubMed:12759376).
  Related references:
  PMID:11751411
  PMID:11950885
  PMID:12759376
• Additional features include alternative splicing, a coiled-coil domain, transmembrane helices, GTP binding, and ubiquitylation modifications.

Experimental Guidance and Technical Tips

• The immunogen was raised against amino acids 622–741 of human MFN1; confirm sequence conservation in mouse and rat for cross-reactivity.
• For western blot, a band around 84 kDa is expected; optimize loading controls and consider mitochondrial enriched lysates.
• In immunofluorescence/ICC, validate mitochondrial localization by colocalization with established mitochondrial markers.
• ELISA applications may require optimization of coating conditions and antibody dilution; consider using recombinant MFN1 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering reliable access to antibodies like this anti-MFN1 polyclonal antibody to support mitochondrial research and assay development. Our supply capabilities include validated antibody pairs, bulk ancillary reagents, and rare target sourcing, helping diagnostic manufacturers streamline development from concept to clinic.