Anti-Mitofilin Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q16891

Core Product Specifications and Parameters

Biological Background: Mitofilin Function and Localization

• Mitofilin (MICOS complex subunit MIC60) is a core component of the mitochondrial inner membrane organizing system (MICOS) complex, essential for maintaining crista junctions, inner membrane architecture, and forming contact sites between inner and outer mitochondrial membranes, thereby ensuring MICOS stability and proper cristae morphology. Related references: PMID:22114354 PMID:25781180 PMID:32567732
• It localizes to the mitochondrial inner membrane.
• Post-translational modifications include acetylation and phosphorylation, and it is annotated as a host factor involved in viral interactions.
• Alternative names include Cell proliferation-inducing gene 4/52 protein, p87/89, Mic60, and others, reflecting its initial identification in cell proliferation and mitochondrial biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 65-300 of human Mitofilin; consider this region for epitope mapping or competitive binding assays.
• For Western blot, the predicted band is around 84 kDa; include appropriate molecular weight markers and validate with positive controls from human or mouse samples.
• For IHC-P, optimize antigen retrieval and validate in tissue sections known to express Mitofilin.
• In immunoprecipitation, use conditions that preserve the MICOS complex integrity and confirm co-immunoprecipitation of complex members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material sourcing, supporting mitochondrial research and diagnostic development with validated antibodies like this Mitofilin monoclonal antibody. Our supply chain includes optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare-target procurement services to streamline your project from concept to clinic.