Anti-MITF Rabbit Polyclonal Antibody for WB and ELISA - O75030

Core Product Specifications and Parameters

Biological Background: MITF Function and Localization

• Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis.
  Related references: PMID:10587587, PMID:22647378, PMID:27889061
• Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR).
  Related references: PMID:10587587, PMID:22647378, PMID:27889061
• Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation; upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity.
  Related references: PMID:36608670
• Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1).
  Related references: PMID:10587587, PMID:22647378, PMID:27889061
• Critical in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts, and optic cup-derived retinal pigment epithelium.
  Related references: PMID:10587587, PMID:22647378, PMID:27889061
• Localizes to the nucleus, cytoplasm, and lysosome membrane, reflecting its dynamic regulatory roles.
• Expressed in melanocytes (at protein level), retinal pigment epithelium, brain, placenta, and kidney.
  Related references: PMID:9647758, PMID:10578055
• MITF mutations are associated with Waardenburg syndrome type 2A, Tietz syndrome (albinism-deafness), and microphthalmia, highlighting its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-280) targets the N-terminal region of human MITF, which may recognize multiple isoforms if this region is conserved.
• Predicted molecular weight is ~59 kDa; however, UniProt reports a calculated mass of ~58.8 kDa. Post-translational modifications may cause band shifts in WB.
• For Western blot, standard protocols for polyclonal antibodies can be applied. Validate the antibody in relevant cell or tissue lysates expressing MITF (e.g., melanocyte lines, retinal pigment epithelium).
• For ELISA, consider using recombinant MITF protein or peptide as capture antigen; titer and specificity should be determined experimentally.
• Cross-reactivity with mouse indicates potential use in mouse models, but validation in mouse tissue extracts is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop source for IVD raw materials, providing diagnostic manufacturers and research labs with reliable access to validated antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-MITF rabbit polyclonal antibody supports research into melanocyte development, pigmentation disorders, and related diseases. Our comprehensive portfolio covers every stage from concept to clinic, ensuring streamlined procurement for your IVD development needs.