Anti-MITF Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O75030

Core Product Specifications and Parameters

Biological Background: MITF Function and Localization

• MITF (Microphthalmia-associated transcription factor) is a Class E basic helix-loop-helix protein (bHLHe32) that functions as a master regulator of melanocyte survival, differentiation, and melanosome biogenesis. Related references: PMID:10587587, PMID:22647378, PMID:27889061
• It binds to M-box (5'-TCATGTG-3') and E-box (5'-CACGTG-3') DNA elements in the promoters of pigmentation genes, including tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1), thereby regulating melanin synthesis. Related references: PMID:10587587, PMID:22647378, PMID:27889061
• MITF activity is coupled to nutrient sensing: in the presence of amino acids, MTOR-mediated phosphorylation inactivates MITF, whereas starvation or lysosomal stress leads to its dephosphorylation and activation, linking metabolism to transcription. Related references: PMID:36608670
• The protein localizes mainly to the nucleus but can also be found in the cytoplasm and on the lysosome membrane, indicative of multiple regulatory compartments.
• Expression is highest in melanocytes and retinal pigment epithelium, with additional presence in brain, placenta, and kidney. Related references: PMID:9647758, PMID:10578055
• Multiple isoforms are generated by alternative splicing, and mutations in the MITF gene are linked to Waardenburg syndrome type 2A, Tietz albinism-deafness syndrome, and COMMAD syndrome, underscoring its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 402–526) of human MITF, which is conserved and may contribute to the antibody's cross-reactivity with rat MITF.
• For Western blot, an expected band around 59 kDa is predicted based on the canonical isoform; however, alternative splicing may produce additional bands.
• In immunofluorescence/immunocytochemistry, nuclear and potentially cytoplasmic staining should be anticipated given MITF's subcellular localization.
• This rabbit monoclonal format provides high batch-to-batch consistency, making it suitable for reproducible ELISA-based detection methods.
• Perform appropriate controls (e.g., siRNA knockdown or overexpression) to confirm specificity in the relevant tissue or cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our MITF monoclonal antibody supports transcription factor and melanocyte biology research, enabling robust assay development. We partner with you from concept to clinic, ensuring reliable supply and technical consultation.