Anti-MITD1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8WV92

Core Product Specifications and Parameters

Biological Background: MITD1 Function and Localization

• MITD1 (MIT domain-containing protein 1) is a membrane-associated protein encoded by the MITD1 gene in humans.
• It plays a critical role in the final step of cell division, specifically required for efficient abscission at the end of cytokinesis, functioning together with components of the ESCRT-III complex.
• MITD1 localizes to the late endosome membrane, the midbody during cytokinesis, and other membrane structures, reflecting its involvement in membrane trafficking and remodeling.
• Its functional keywords include cell cycle, cell division, endosome, and transport, underscoring its role in membrane dynamics and cellular division.
• The protein has a predicted molecular weight of approximately 29 kDa and is a subject of structural biology studies (3D-structure keyword available).

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the N-terminal region (amino acids 1–249) of human MITD1; it should recognize the full-length protein in Western blot analysis, with an expected band around 29 kDa.
• For WB, consider using whole-cell lysates from human, mouse, or rat cell lines as positive controls, and validate the band size with molecular weight markers.
• In IHC-P, antigen retrieval methods may be required to expose the epitope; nuclear and membrane staining patterns may be observed depending on the cellular context and fixation conditions.
• ELISA applications can be optimized using the recombinant immunogen or peptide antigens; cross-reactivity with mouse and rat suggests suitability for multi-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with high-quality IVD raw materials, including validated antibodies like this MITD1 polyclonal antibody for studies in cell division and membrane trafficking. We offer a comprehensive portfolio of antibody pairs, monoclonal and polyclonal options, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.