Anti-MIPEP Rabbit Polyclonal Antibody for WB, ELISA - Q99797

Core Product Specifications and Parameters

Biological Background: MIPEP Function and Localization

• MIPEP (mitochondrial intermediate peptidase) is a metalloprotease responsible for cleaving nuclear-encoded proteins imported into the mitochondrion, maturing them to their functional sizes.
• This processing is essential for the proper assembly and activity of the mitochondrial proteome.
• The enzyme localizes exclusively to the mitochondrial matrix, consistent with its role in post-import protein maturation.
• It is classified as a metalloprotease that requires divalent metal ions for catalysis, with evidence supporting binding to calcium, cobalt, iron, magnesium, manganese, and zinc.
• Post-translational modification includes acetylation.
• Mutations in MIPEP are associated with primary mitochondrial disease, specifically combined oxidative phosphorylation deficiency 31 (COXPD31).
• The protein possesses a transit peptide that directs its own import into mitochondria, after which it is cleaved to form the mature protease.
• The predicted molecular weight of the full-length precursor is approximately 81 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment corresponding to amino acids 511-713, which lies in the C-terminal region of the mature protein; this design may favor detection of processed MIPEP.
• For Western blot, consider using mitochondrial-enriched lysates to improve signal, given the mitochondrial matrix localization.
• ELISA applications may be optimized by testing both direct and sandwich formats, depending on the sample matrix and available antibody pairs.
• Cross-reactivity with mouse and rat has been validated by the supplier, but we recommend verifying species-specific reactivity in your experimental system.
• The observed band may appear near 81 kDa, but note that the mature, processed form may be slightly smaller; always compare with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

For diagnostic manufacturers and researchers requiring validated IVD raw materials, CamelBio provides streamlined access to antibodies like MIPEP Rabbit pAb, along with complementary reagents for mitochondrial research. Our procurement platform offers monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, ensuring you can obtain all necessary components from a single partner. Whether you need antibody pairs, optimized detection reagents, or customized raw-material solutions for mitochondrial disease studies, we bridge the gap from concept to clinic.