Anti-MIPEP Monoclonal Antibody for WB, IF/ICC, ELISA - Q99797

Core Product Specifications and Parameters

Biological Background: Mitochondrial Intermediate Peptidase (MIPEP) Function and Localization

• MIPEP, also known as MIP, HMIP, and COXPD31, is a mitochondrial intermediate peptidase that processes nuclear-encoded mitochondrial precursor proteins after their import.
• It localizes specifically to the mitochondrial matrix, where it cleaves N-terminal octapeptides from intermediate forms of proteins to generate their mature, functional sizes.
• MIPEP is a zinc-dependent metalloprotease that requires divalent cations such as Zn²⁺, Mn²⁺, or Mg²⁺ for catalytic activity.
• The protein belongs to the M3 family of metalloendopeptidases and is highly conserved across species, reflecting its essential role in mitochondrial proteostasis.
• Mutations in the MIPEP gene cause combined oxidative phosphorylation deficiency 31 (COXPD31), a severe mitochondrial disease with multisystem involvement.
• Post-translational modifications such as acetylation may regulate MIPEP activity or stability within the mitochondrial matrix.
• As a key enzyme in mitochondrial protein maturation, MIPEP impacts respiratory chain assembly, ATP production, and overall cellular energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 511–713 of human MIPEP; the antibody may recognize the full-length protein (~81 kDa). Verify specificity using mitochondrial-enriched lysates from human cell lines (e.g., HeLa, HEK293) and consider siRNA knockdown controls.
• For Western blot, optimize extraction buffers to preserve mitochondrial proteins and use fresh or properly stored samples to avoid degradation.
• In IF/ICC, combine with a mitochondrial marker such as Tom20 for unambiguous localization. Permeabilization steps should be optimized for mitochondrial membrane antigens.
• ELISA applications may be feasible for native or recombinant MIPEP, but empirical optimization of coating and detection conditions is recommended.
• As a rabbit monoclonal antibody, it offers consistent lot-to-lot reproducibility and high affinity, suitable for demanding quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. For mitochondrial disease research, we supply validated antibody pairs, optimized monoclonal antibodies like this anti-MIPEP clone, and bulk ancillary reagents. Tell us your specific target or assay requirement, and we'll design a custom sourcing strategy to accelerate your project.