Anti-MIP Polyclonal Antibody for WB, IHC-P, ELISA - P30301

Core Product Specifications and Parameters

Biological Background: MIP Function and Localization

• MIP (Aquaporin-0) forms homotetrameric transmembrane channels that mediate water transport along osmotic gradients, crucial for lens water homeostasis. Related references: PMID:11001937, PMID:24120416
• Beyond water transport, MIP acts as a cell-to-cell adhesion molecule, forming thin junctions between lens fiber cells, which is essential for maintaining lens structure and transparency. Related references: PMID:24120416
• The protein is localized to the cell membrane and cell junctions of lens fiber cells.
• Tissue specificity: Expressed in the cortex and nucleus of the retina lens (at protein level) and is a major component of lens fiber gap junctions. Related references: PMID:30790544, PMID:24120416
• Keywords and disease associations: MIP is implicated in cataract formation, with disease variants reported. It is a lipoprotein, subject to palmitoylation and phosphorylation, and belongs to the aquaporin family.
• The calculated molecular weight is approximately 28 kDa, consistent with its transmembrane helix structure.

Experimental Guidance and Technical Tips

• For Western blot (WB), a single band around 28 kDa representing the MIP monomer is expected. Consider using reducing conditions and validate in your specific sample system.
• In immunohistochemistry (IHC-P), MIP is highly expressed in lens fiber cells; include appropriate positive and negative controls to confirm specificity.
• For ELISA, the immunizing peptide (amino acids 50-150) can be used as a positive control to assess antibody reactivity. Titrate the antibody for optimal signal.
• Given the high sequence conservation among human, mouse, and rat, this antibody is suitable for cross-species studies; however, validation in the target species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials. This anti-MIP polyclonal antibody exemplifies our commitment to providing high-quality immunological reagents for aquaporin and lens biology research. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your development pipeline from concept to clinic.