Anti-MIOX Polyclonal Antibody for WB, IF-P, ELISA - Q9UGB7

Core Product Specifications and Parameters

Biological Background: Inositol Oxygenase Function and Localization

• MIOX, also known as aldehyde reductase-like 6 (ALDRL6), kidney-specific protein 32 (KSP32), and renal-specific oxidoreductase (RSOR), is an enzyme encoded by the MIOX gene in humans (Gene ID: 55586).
• It is classified as an oxidoreductase and contains iron-binding sites, indicating metal-dependent catalytic activity.
• Subcellular localization studies show MIOX is primarily cytoplasmic.
• Tissue specificity: MIOX expression is kidney-specific.
• Alternative splicing of MIOX generates multiple transcript variants, as indicated by UniProt keyword.
• Proteomics identification confirms its presence in reference proteome datasets.
• Phosphoprotein status suggests post-translational regulation.

Experimental Guidance and Technical Tips

• The antibody was produced by immunizing rabbits with a recombinant fusion protein covering amino acids 1–285 of human MIOX (NP_060054.4), which may result in recognition of multiple epitopes.
• For Western blot (WB), the predicted molecular weight of MIOX is ~33 kDa; consider using a suitable molecular weight marker and loading control.
• In immunofluorescence (IF-P), verify fixation and permeabilization protocols to preserve cytoplasmic localization of the target.
• For ELISA, titrate the antibody to determine optimal working concentrations for antigen detection.
• The antibody cross-reacts with mouse and rat according to supplier specifications; validate species reactivity in your specific sample system before critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a wide range of IVD raw materials. This MIOX polyclonal antibody supports kidney biology and oxidoreductase research, complementing our portfolio of validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Leverage our integrated supply chain to accelerate your project from concept to clinic.