Anti-MIOS (MIO, Sea4, Yulink) Polyclonal Antibody for WB, ELISA - Q9NXC5

Core Product Specifications and Parameters

Biological Background: MIOS Function and Localization

• MIOS, also known as Missing oocyte meiosis regulator homolog, is a core component of the GATOR2 complex, a key regulatory hub of the mTORC1 signaling pathway. Related references: PMID:23723238, PMID:26586190, PMID:27487210
• Within the GATOR2 complex, MIOS functions as an activator of amino acid-sensing mTORC1 signaling by inhibiting the GATOR1 subcomplex, thereby relieving mTORC1 suppression. Related references: PMID:23723238, PMID:26586190, PMID:27487210
• The GATOR2 complex, including MIOS, acts as an E3 ubiquitin-protein ligase that ubiquitinates the NPRL2 component of GATOR1, leading to GATOR1 inactivation under amino acid-rich conditions. Related references: PMID:36528027
• In contrast, during amino acid deprivation, GATOR2 activity is inhibited, allowing GATOR1 to suppress mTORC1 signaling, a process that involves MIOS-mediated regulation. Related references: PMID:25263562, PMID:25457612, PMID:26586190
• MIOS is crucial for preventing autoubiquitination of WDR24, the catalytic subunit of the GATOR2 complex, thereby maintaining complex stability and function. Related references: PMID:35831510
• The protein localizes predominantly to the lysosomal membrane, positioning the GATOR2 complex at a critical cellular signaling node.
• MIOS contains several structural features, including WD repeats and a zinc-finger domain, and is subject to alternative splicing and phosphorylation.
• The GATOR2 complex, through MIOS, is required for brain myelination, highlighting its role in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 556–875 of human MIOS, a region likely accessible for antibody recognition in denaturing and native conditions, supporting its use in WB and ELISA.
• For Western blotting, consider optimizing blocking and primary antibody dilution in your specific sample matrix to reduce background; the expected band size is approximately 99 kDa.
• Validate cross-reactivity in mouse and rat samples using appropriate positive controls, as the immunogen sequence may exhibit high homology across these species.
• For ELISA applications, titrate the antibody in both direct and sandwich formats to determine working concentrations suitable for your assay sensitivity requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a complete portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies like this anti-MIOS antibody, which targets the mTOR signaling pathway relevant to oncology and neuroscience. We offer bulk ancillary reagents, custom antibody pair development, and rare target sourcing to streamline your assay development from concept to clinic. Partner with CamelBio for efficient, integrated procurement and technical consultation tailored to your diagnostic needs.