Anti-Mineralocorticoid receptor (NR3C2) Rabbit pAb - P08235

Core Product Specifications and Parameters

Biological Background: Mineralocorticoid receptor Function and Localization

• Mineralocorticoid receptor (MR, NR3C2) is a nuclear receptor that mediates the effects of mineralocorticoids like aldosterone and glucocorticoids such as cortisol. It binds to mineralocorticoid response elements (MREs) in DNA to regulate gene transcription.
• Activation of MR increases ion and water transport in epithelial tissues, thereby raising extracellular fluid volume and blood pressure while lowering serum potassium levels.
• MR belongs to the nuclear receptor subfamily 3 group C, featuring a zinc-finger DNA-binding domain and a C-terminal steroid-binding domain.
• The receptor is expressed ubiquitously, with highest levels in kidney distal tubules, cortical collecting ducts, and sweat glands; lower expression is found in cardiomyocytes, epidermis, and colon enterocytes.
• Subcellularly, MR shuttles between the cytoplasm and nucleus, and is also associated with the endoplasmic reticulum membrane upon ligand activation.
• Post-translational modifications include phosphorylation, and alternative splicing generates isoforms with potentially distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 450-600) of human MR, which may recognize multiple isoforms. For Western blot, a band around 107 kDa is expected.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), appropriate controls (e.g., knockout tissue, blocking peptide) should be included to confirm specificity.
• This antibody is also applicable in ELISA; consider optimizing coating and detection conditions for the specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, offering validated antibodies, recombinant proteins, and ancillary reagents to support diagnostic development. Our portfolio includes reliable anti-MR antibodies suitable for mineralocorticoid signaling and hypertension research. Leverage our one-stop supply chain to streamline your transition from concept to clinical application.