Anti-MIIP Rabbit Polyclonal Antibody for WB, ELISA - Q5JXC2

Core Product Specifications and Parameters

Biological Background: MIIP Function and Localization

• MIIP (Migration and invasion-inhibitory protein), also known as IIP45 or IGFBP2-binding protein, is encoded by the MIIP gene and produces at least two isoforms via alternative splicing.
• The protein functions as an inhibitor of glioma cell invasion by down-regulating adhesion- and motility-associated genes such as NFKB2 and ICAM1, exhibiting effects that oppose those of IGFBP2 on cell invasion.
• Isoform 1 is expressed in normal brain but underexpressed in glioma tissues at the protein level, while isoform 2 is not detected in normal organs but is expressed in gliomas, with increasing levels correlating with glioma progression.
• Despite transcript expression in gliomas, isoform 2 appears unstable at the protein level in glioma cells, suggesting post‑translational regulation.
• MIIP is classified as a phosphoprotein and has been identified in proteomics studies as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a fragment (aa 279-388) of human MIIP; consider potential detection of multiple isoforms if the epitope is conserved.
• For Western blot analysis, validate antibody specificity in appropriate glioma cell lines or tissue lysates known to express MIIP.
• In ELISA applications, ensure proper positive and negative controls, and consider testing cross-reactivity with rat samples as annotated.
• As a polyclonal antibody, lot‑to‑lot consistency should be confirmed for quantitative studies, and optimal dilutions must be determined empirically.

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