Anti-MIF Polyclonal Antibody for WB, IF/ICC, ELISA - P14174

Core Product Specifications and Parameters

Biological Background: MIF Function and Localization

• MIF, also designated as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, and phenylpyruvate tautomerase, is encoded by the MIF gene (GLIF, MMIF).
• As a pro-inflammatory cytokine, MIF participates in innate immunity against bacterial pathogens and modulates macrophage activity during host defense. Related references: PMID:15908412 PMID:17443469 PMID:23776208
• MIF counteracts the anti-inflammatory effects of glucocorticoids. Related references: PMID:15908412 PMID:17443469 PMID:23776208
• The protein exhibits in vitro tautomerase activity towards phenylpyruvate and dopachrome, although the physiological significance of these activities is not firmly established. Related references: PMID:11439086 PMID:17526494
• Subcellularly, MIF is localized to both the cytoplasm and the secretory pathway.
• Key post-translational modifications include acetylation, and the protein is a member of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human MIF (aa 1-115); the polyclonal antibody may recognize both native and denatured protein conformations.
• For Western blotting, consider using whole cell lysates from human, mouse, or rat tissues/cells, given the reported cross-reactivity.
• For immunofluorescence/ICC, validate the antibody in relevant cell lines under appropriate fixation and permeabilization conditions.
• ELISA applications could be explored for quantitative detection of MIF; note that MIF is a secreted protein, so culture supernatants may be a suitable sample type.
• As with any polyclonal antibody, lot-to-lot consistency should be verified; consider including appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio supports your entire diagnostic development pipeline with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-MIF polyclonal antibody is a valuable tool for research into innate immunity, inflammation, and infectious disease, and CamelBio can assist with custom orders, technical consultation, and supply chain solutions to accelerate your project from concept to clinic.