Anti-MIF Monoclonal Antibody for WB, IHC-P, ELISA - P14174

Core Product Specifications and Parameters

Biological Background: MIF Function and Localization

• Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, or phenylpyruvate tautomerase, is encoded by the MIF gene (GLIF, MMIF).
• Functions as a pro-inflammatory cytokine in innate immunity, regulating macrophage activity and counteracting the anti-inflammatory effects of glucocorticoids. Related references: PMID:15908412 PMID:17443469 PMID:23776208
• Exhibits phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro, though the physiological substrate and functional relevance remain unclear. Related references: PMID:11439086 PMID:17526494
• Localizes to the cytoplasm and is secreted.
• Post-translationally acetylated; molecular weight approximately 12.5 kDa (UniProt: 12476 Da).
• Associated keywords: Immunity, inflammatory response, innate immunity, isomerase, acetylation.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 114-199 of human MIF (NP_002406.1). Consider using it for detecting the full-length protein.
• For Western blot (WB), anticipate a band near 12 kDa; optimize sample loading and detection conditions in your specific experimental system.
• For immunohistochemistry (IHC-P) and ELISA, validate the antibody in relevant tissue or sample types from human, mouse, or rat.
• Cross-reactivity with human, mouse, and rat MIF is indicated; if using other species, perform homology analysis and confirm reactivity empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering validated monoclonal antibodies, optimized antibody pairs, and ancillary reagents to support inflammation and innate immunity research. This anti-MIF antibody exemplifies our commitment to providing high-quality reagents for diagnostic development. From concept to clinic, CamelBio bridges your sourcing needs with reliable supply and technical expertise.