Anti-MIER1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8N108

Core Product Specifications and Parameters

Biological Background: MIER1 Function and Localization

• MIER1 (Mesoderm induction early response protein 1) is a transcriptional repressor that regulates gene expression by recruiting histone deacetylase 1 (HDAC1) to target promoters, leading to chromatin silencing.
• It suppresses SP1-dependent transcription, influencing genes involved in cell growth and differentiation.
• Multiple protein isoforms are produced by alternative splicing, potentially with distinct functional properties.
• Subcellular localization is predominantly nuclear, though cytoplasmic presence has also been reported.
• MIER1 is ubiquitously expressed at low levels, with relatively higher expression in heart, testis, thyroid, ovary, and adrenal gland; its transcripts are significantly upregulated in breast carcinoma cell lines and tumors.
• Post-translational modifications include phosphorylation and isopeptide bond formation via ubiquitin-like conjugation, which may modulate its activity and stability.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment spanning amino acids 110–160 of human MIER1; it is expected to recognize all isoforms containing this epitope.
• For Western blotting, a primary band around 58 kDa (predicted molecular weight) is anticipated; consider using a reduced sample preparation and appropriate blocking conditions.
• For immunohistochemistry (paraffin), antigen retrieval may be required; pilot testing of dilution series is recommended for optimal signal-to-noise ratio.
• For ELISA applications, the antibody can be used as a capture or detection reagent after appropriate pairing and concentration optimization; cross-reactivity with mouse samples allows flexible model system usage.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, laboratories, and research institutions with high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like MIER1—a transcriptional repressor implicated in cancer—we offer one-stop sourcing support from concept to clinic, helping you accelerate assay development and secure reliable raw material supply.