Anti-MIEN1 Rabbit Polyclonal Antibody for WB, ELISA - Q9BRT3

Core Product Specifications and Parameters

Biological Background: MIEN1 Function and Localization

• MIEN1 (Migration and invasion enhancer 1), also known as C35, HBV X-transactivated gene 4 protein, or XTP4, is a 12 kDa protein encoded by the MIEN1 gene (GeneID 84299) on chromosome 17.
• MIEN1 promotes cell migration by inducing filopodia formation at the leading edge of migrating cells.
• MIEN1 is involved in the regulation of apoptosis, possibly through modulation of caspase-3 (CASP3) activity, and may participate in redox-related processes (UniProt keyword: Redox-active center).
• The protein is localized to the cytoplasm, cytosol, and cell membrane, consistent with its roles in signaling and cytoskeletal remodeling.
• Post-translational modifications include acetylation and prenylation, and it contains a disulfide bond, classifying it as a lipoprotein with a redox-active center.
• Under normal conditions, MIEN1 expression is restricted to Leydig cells, but it is strongly up-regulated in breast cancers, brain cancer distant metastases, and higher-grade prostate adenocarcinomas, highlighting its potential as a cancer biomarker.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human MIEN1 protein (amino acids 1-115), providing broad epitope coverage for western blot detection of the 12 kDa band. Consider using a low molecular weight range gel or prolonged transfer for optimal detection.
• For ELISA, the antibody may be used as a capture or detection reagent after pairing validation. Check cross-reactivity with mouse or rat MIEN1 if needed, as this antibody is human-specific based on specifications.
• Given MIEN1's cancer-associated upregulation, consider using human cancer cell lines (e.g., breast or prostate cancer) as positive controls for WB. Loading controls should include proteins of similar size.
• Due to the protein's low molecular weight, consider using high-sensitivity chemiluminescent substrates and avoid over-washing during WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including this anti-MIEN1 polyclonal antibody for cancer and apoptosis research. Our catalog features validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Partner with CamelBio to accelerate your assay development from concept to clinic.