Anti-Midkine (MDK) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P21741

Core Product Specifications and Parameters

Biological Background: Midkine (MDK) Function and Localization

• Midkine is a secreted cytokine and growth factor that signals through cell-surface proteoglycan and non-proteoglycan receptors, regulating cell proliferation, survival, migration, and differentiation. Related references: PMID:10212223, PMID:10772929, PMID:12084985
• It mediates inflammatory responses by recruiting neutrophils and macrophages to sites of inflammation via cooperation with ITGB2 and LRP1, inducing chemokine expression; it also suppresses tolerogenic dendritic cells, inhibiting regulatory T cell differentiation while promoting Th1 cell differentiation. Related references: PMID:10683378, PMID:24458438, PMID:22323540
• Midkine promotes tissue regeneration after injury, including heart, liver, and bone, by modulating inflammatory cell recruitment, activating anti-apoptotic MAPK and AKT pathways, and promoting angiogenesis.
• In the nervous system, it promotes neuronal survival and neurite outgrowth by binding to receptors like PTPRZ1, SDC3, GPC2, and LRP1, and it facilitates neuronal migration. Related references: PMID:10212223, PMID:12084985, PMID:1768439, PMID:10772929
• Midkine binds anaplastic lymphoma kinase (ALK), activating MAPK and PI3K pathways to induce cell proliferation, and it promotes epithelial-to-mesenchymal transition via NOTCH2. Related references: PMID:12122009, PMID:18469519
• It plays a role in vascular biology by inducing VEGFA expression and nitric oxide synthase, promoting vasodilation and endothelial proliferation during arteriogenesis.
• Midkine negatively regulates bone formation in response to mechanical load by inhibiting Wnt/β-catenin signaling in osteoblasts.
• The protein is secreted and is expressed in various tumor cell lines; in insulinoma tissue it is predominantly found in precancerous lesions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23-143 of human MDK, covering the mature protein after signal peptide cleavage; this design may favor detection of the secreted form.
• For Western blot (WB), consider running samples under reducing conditions and validate in tissues or cell lines known to express midkine, such as tumor-derived lines.
• For immunofluorescence/immunocytochemistry (IF/ICC), include appropriate controls and optimize fixation/permeabilization for secreted proteins; validation in relevant cell types is recommended.
• ELISA may be suitable for quantitative detection of midkine in serum, plasma, or culture supernatants; pairing with a matched detection antibody can improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, offering a comprehensive portfolio of antibodies and ancillary reagents for research areas such as cytokine biology, inflammation, and regenerative medicine. Alongside this anti-Midkine polyclonal antibody, we can supply validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development. Our expert team also assists in sourcing rare-target raw materials to cover your entire development pipeline, from concept to clinic.