Anti-MICU2 Rabbit Polyclonal Antibody for WB & ELISA - Q8IYU8

Core Product Specifications and Parameters

Biological Background: MICU2 Function and Localization

• MICU2 (Calcium uptake protein 2, mitochondrial; EF-hand domain-containing family member A1) functions as a calcium sensor of the mitochondrial calcium uniporter (MCU) channel, detecting calcium levels through its EF-hand domains.
  Related references:
  PMID:24503055 PMID:24560927 PMID:26903221

• It forms a disulfide-linked heterodimer with MICU1 that dually regulates MCU activity: inhibiting at low calcium and stimulating at high calcium.
  Related references:
  PMID:24560927 PMID:26903221 PMID:28615291

• At low cytosolic calcium concentrations, MICU1 occludes the MCU pore, preventing mitochondrial calcium uptake; calcium binding to both MICU1 and MICU2 induces a conformational change that moves the heterodimer away from the pore, allowing calcium permeation.
  Related references:
  PMID:32494073 PMID:32667285 PMID:32762847

• Subcellular localization is primarily within the mitochondrial intermembrane space and inner membrane, where it associates with the MCU complex.

• The protein contains characteristic EF-hand motifs, consistent with its role in calcium sensing.

• Phosphorylation has been identified as a post-translational modification that may regulate its function or interactions.

• As a key component of mitochondrial calcium homeostasis, MICU2 is essential for cellular energy metabolism, apoptosis, and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody is a synthetic peptide spanning amino acids 200–300 of human MICU2. This region may be accessible for antibody binding and contributes to target specificity.
• For Western blot, consider testing a dilution range (e.g., 1:500–1:2000) using human cell lysates that express MICU2. The predicted molecular weight is approximately 50 kDa, although post-translational modifications or mitochondrial targeting sequences might alter migration.
• In ELISA, the antibody can be employed as a capture or detection reagent; optimization of coating antigen and antibody concentrations is recommended.
• As this is a polyclonal antibody, lot-to-lot variability can occur; always perform independent validation in the specific sample system.
• Because MICU2 functions in a heterodimeric complex, co-immunoprecipitation or proximity ligation assays may be considered to study protein interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from early-stage development to clinical application. This anti-MICU2 antibody supports research into mitochondrial calcium signaling, metabolism, and related pathophysiological conditions. We provide one-stop access to validated antibody pairs, recombinant proteins, and ancillary reagents, along with technical services to accelerate your assay development and rare target sourcing needs.