Anti-MICU1 Rabbit Polyclonal Antibody for WB, ELISA - Q9BPX6

Core Product Specifications and Parameters

Biological Background: MICU1 (Calcium uptake protein 1, mitochondrial) Function and Localization

• MICU1 functions as the calcium sensor of the mitochondrial calcium uniporter (MCU) channel, sensing calcium levels via its EF-hand domains. Related references: PMID:20693986, PMID:23101630, PMID:23747253
• MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates or inhibits MCU activity depending on calcium concentration. Related references: PMID:24560927, PMID:26903221, PMID:28615291
• At low calcium, MICU1 occludes the MCU pore, preventing calcium uptake; at higher calcium, calcium binding induces a conformational change that moves MICU1-MICU2 away, allowing permeation. Related references: PMID:32494073, PMID:32667285, PMID:32762847
• An alternative isoform of MICU1 with higher calcium affinity is expressed in skeletal muscle, promoting rapid mitochondrial calcium uptake to sustain ATP production during strenuous exercise.
• MICU1 also protects against manganese toxicity by maintaining MCU pore occlusion upon manganese binding, which fails to induce the activating conformational change. Related references: PMID:30082385, PMID:30403999
• MICU1 acts as a barrier for MCU inhibitors like ruthenium red and Ru360. Related references: PMID:37244260
• It regulates mitochondrial cristae structure independently of calcium uniporter activity. Related references: PMID:31427612, PMID:37098122
• MICU1 localizes to the mitochondrial intermembrane space and inner membrane and is expressed in epithelial cell lines, epidermal keratinocytes, and dermal endothelial cells.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 105–255 within the N-terminal EF-hand domains; consider potential isoform detection differences when interpreting results.
• For Western blotting, the predicted molecular weight is ~54 kDa; validate the band using mitochondrial-enriched fractions or whole cell lysates from human, mouse, or rat samples.
• For ELISA applications, the antibody may be suitable for capturing or detecting recombinant MICU1 or endogenous protein in cell/tissue extracts; optimize coating and detection conditions accordingly.
• As a polyclonal antibody, batch-to-batch consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers a streamlined sourcing solution for IVD raw materials, including validated polyclonal antibodies such as this Anti-MICU1 antibody. Beyond antibodies, our portfolio encompasses recombinant proteins, bulk ancillary reagents, and customized rare-target sourcing to support your mitochondrial calcium signaling and immunoassay development needs.