Anti-MIB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96AX9

Core Product Specifications and Parameters

Biological Background: MIB2 Function and Localization

• MIB2, also known as mind bomb homolog 2, skeletrophin, or ZZANK1, is an E3 ubiquitin-protein ligase and a key regulator of the Notch signaling pathway.
• It functions by mediating the ubiquitination of Delta receptors, the ligands for Notch proteins, thereby promoting their endocytosis and positively regulating Notch signaling.
• MIB2 belongs to the mind bomb family of RING-type E3 ubiquitin transferases and contains multiple structural motifs including ankyrin repeats, zinc finger ZZ type, and RING finger domains.
• Subcellularly, MIB2 localizes to the cytoplasm and endosomes, consistent with its role in receptor trafficking and endocytosis.
• Tissue expression analysis indicates MIB2 is predominantly expressed in skeletal muscle, with lower levels detected in heart, brain, and kidney.
• Post-translational modifications of MIB2 include acetylation and phosphorylation, which may modulate its activity and interactions within ubiquitination pathways.
• Additional functional annotations suggest MIB2 may have actin-binding properties, possibly linking it to cytoskeletal dynamics.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight is approximately 104 kDa. Validate the antibody using a positive control lysate from cells expressing MIB2, and use an appropriate molecular weight marker.
• In immunofluorescence (IF/ICC), since MIB2 localizes to the cytoplasm and endosomes, optimize fixation and permeabilization conditions to preserve these structures. Counterstaining with organelle markers may help confirm localization.
• For ELISA applications, consider using recombinant MIB2 protein or cell lysates as the coating antigen, and titrate the antibody to determine optimal working concentrations.
• As a polyclonal antibody, slight lot-to-lot variations may occur; we recommend confirming performance in your specific assay system with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of validated antibodies, bulk ancillary reagents, and custom procurement services to support diagnostic development from concept to clinic. For researchers and manufacturers focused on the Notch signaling pathway or ubiquitination mechanisms, CamelBio can provide additional antibody pairs, optimized monoclonal alternatives, or rare target raw materials to streamline your workflow. Contact us to explore how we can support your specific project needs.