Anti-MHC Class II (I-A/I-E) Monoclonal Antibody for Flow Cytometry - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• MHC class II molecules are heterodimeric glycoproteins composed of alpha and beta chains, presenting extracellular peptides to CD4+ T cells. The I-A and I-E isotypes are the classical MHC II molecules in mouse.
• The I-A beta chain (encoded by H2-Ab1) and I-E beta chain (encoded by H2-Eb1) are highly polymorphic membrane proteins that form complexes with their respective alpha chains, I-A alpha and I-E alpha.
• These molecules are critical for adaptive immunity, mediating antigen presentation to T-helper cells and shaping T-cell repertoire during thymic selection.
• Subcellular localization: Predominantly expressed on the plasma membrane of professional antigen-presenting cells (dendritic cells, B cells, macrophages) and thymic epithelial cells.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and ubiquitination, which regulate MHC II trafficking and turnover.
• Keywords: 3D-structure, Adaptive immunity, Disulfide bond, Glycoprotein, Immunity, Membrane, MHC II, Reference proteome, Signal, Transmembrane, Transmembrane helix, Ubl conjugation.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Include appropriate isotype control and viability dye to discriminate dead cells, which may exhibit non-specific binding.
• Validate antibody performance in relevant mouse strains; note that some allotype-specific variations may affect recognition.
• Perform surface staining on live, unfixed cells; if fixation is required, test for potential epitope masking.
• Use fluorochrome-matched compensation controls when performing multicolor flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and sourcing solutions, including validated antibodies, bulk ancillary reagents, and custom procurement services. As a one-stop partner for diagnostic manufacturers and research labs, we can support your MHC Class II and immunology-related projects from concept to clinic. Contact us to explore how our supply chain capabilities can accelerate your development timelines.