Anti-MHC Class II (I-A/I-E) Monoclonal Antibody for FC, ELISA - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II Function and Localization

• The H-2 class II histocompatibility antigen, I-A beta chain (encoded by H2-Eb1) is a transmembrane glycoprotein of the MHC class II family.
• It localizes to the cell membrane, consistent with its role in presenting peptides to immune cells.
• This protein participates in adaptive immunity, a critical pathway for pathogen defense.
• Structural features include a signal peptide, a transmembrane helix, and disulfide bonds that stabilize the αβ heterodimer.
• Post-translational modifications encompass glycosylation and ubiquitin-like conjugation (Ubl conjugation).
• The processed mature protein has a calculated molecular weight of approximately 30.2 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein encompassing multiple MHC Class II variants; it may detect I-A and I-E alleles.
• For flow cytometry, consider using cell surface staining protocols with appropriate isotype controls and verify the staining pattern in mouse splenocytes or antigen-presenting cells.
• In ELISA applications, titrate the antibody and validate binding to the recombinant immunogen or native MHC class II complexes in the appropriate sample matrix.
• Although specificity for mouse MHC II is reported, perform cross-reactivity checks if using samples from other species.

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