Anti-MGRN1 Polyclonal Antibody for WB, IF/ICC, ELISA - O60291

Core Product Specifications and Parameters

Biological Background: MGRN1 Function and Localization

• MGRN1 (Mahogunin RING finger protein 1, also known as RNF156) is an E3 ubiquitin-protein ligase that contains a RING finger domain and mediates monoubiquitination of target proteins.
• Mediates monoubiquitination of TSG101 in the presence of UBE2D1, but not UBE2G1 or UBE2H. Related references: PMID:17229889 PMID:19703557
• Plays a critical role in the regulation of endosome-to-lysosome trafficking. Related references: PMID:17229889
• Impairs MC1R- and MC4R-mediated signaling by competing with GNAS for binding to melanocortin receptors, thereby inhibiting agonist-induced cAMP production; does not inhibit ADRB2 signaling. Related references: PMID:19737927
• Acts as a negative regulator of hedgehog signaling (by similarity).
• Subcellularly localizes to early endosomes, cytoplasm, cytosol, nucleus, and cell membrane.
• Features zinc-finger motifs, potential myristoylation and phosphorylation sites, and undergoes alternative splicing to produce multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human MGRN1 protein (amino acids 1-538), providing broad epitope coverage that may enhance detection of endogenous MGRN1 in various assay formats.
• For Western blot (WB), consider starting with a typical polyclonal antibody dilution range and optimize using your specific sample lysates; the predicted molecular weight is ~61 kDa.
• In immunofluorescence/immunocytochemistry (IF/ICC), validate fixation and permeabilization protocols to preserve the multi-compartment localization of MGRN1.
• Given confirmed cross-reactivity with mouse and rat, this antibody may be suitable for orthologous studies; verify specificity in the relevant species beforehand.
• For ELISA applications, pairing with a matched detection antibody may improve assay sensitivity; test both direct and sandwich ELISA formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and research institutions with a one-stop supply of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For ubiquitination and endosomal trafficking research focused on targets like MGRN1, we offer optimized polyclonal antibodies and can assist in sourcing rare raw materials to support your assay development needs. Whether you require ready-to-use reagents or custom procurement solutions, CamelBio bridges the gap from concept to clinic.