Anti-MGP Rabbit Polyclonal Antibody for WB, IHC-P, IF-P, ELISA - P08493

Core Product Specifications and Parameters

Biological Background: Matrix Gla protein (MGP) Function and Localization

• Matrix Gla protein (MGP), also known as cell growth-inhibiting gene 36 protein (GIG36), is a 12.3 kDa secreted protein encoded by the MGP gene.
• Contains vitamin K-dependent gamma-carboxyglutamic acid (Gla) residues that confer high affinity for calcium and hydroxyapatite, critical for its function as a mineralization inhibitor.
• Functions as an inhibitor of bone formation and soft-tissue calcification by associating with the organic matrix of bone and cartilage.
• Secreted into the extracellular space, where it regulates chondrogenesis and osteogenesis, as indicated by keyword annotations.
• Post-translationally modified by phosphorylation and disulfide bond formation, contributing to its structural stability and functional activity.
• Alternative splicing produces multiple isoforms, potentially adding to its functional diversity, and its dysfunction is linked to deafness, as noted in the Deafness keyword.

Experimental Guidance and Technical Tips

• The immunogen (aa 20–103) corresponds to the N-terminal portion of mature human MGP, likely excluding the signal peptide; this design targets the processed, secreted form.
• For Western blot, a band around 12 kDa is expected under reducing conditions; however, MGP may form disulfide-linked multimers, so non-reducing conditions could reveal higher molecular weight complexes.
• In IHC-P, antigen retrieval steps may enhance detection, as MGP is embedded in the bone/cartilage matrix; consider using tissues such as bone or calcified cartilage as positive controls.
• Validate cross-reactivity with mouse and rat samples, but note that sequence homology in the immunogen region should be verified for optimal results.
• This polyclonal antibody may exhibit lot-to-lot variation; we recommend including appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a single point of contact for IVD raw-material needs, supplying high-quality antibodies like this anti-MGP polyclonal antibody for bone and mineralization research. Our offerings span validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing, all backed by technical consultation to support your assay development from concept to clinic.