Anti-MGMT Rabbit Polyclonal Antibody for WB, ELISA - P16455

Core Product Specifications and Parameters

Biological Background: MGMT Function and Localization

• MGMT (methylated-DNA--protein-cysteine methyltransferase), also known as O6-methylguanine-DNA methyltransferase, is a key enzyme in the cellular defense against alkylating agents.
• It repairs O6-methylguanine and O4-methylthymine lesions in DNA by stoichiometrically transferring the alkyl group to a cysteine residue in its active site, leading to irreversible inactivation of the enzyme.
• This suicide repair mechanism directly removes mutagenic and cytotoxic adducts, protecting genomic integrity.
• MGMT is a single-copy gene in humans and is primarily localized to the nucleus, consistent with its role in DNA repair.
• The protein binds zinc and contains a metal-binding motif; it is also subject to phosphorylation, which may modulate its activity or stability.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length sequence (residues 1–207) of human MGMT, so the polyclonal antibody may recognize multiple epitopes across the protein.
• For Western blot, the predicted molecular weight is approximately 22 kDa; consider using a nuclear extract or whole-cell lysate from human or mouse cells that express MGMT.
• Validate the antibody’s reactivity in your specific experimental system, as expression levels of MGMT can vary widely among cell types and tissues.
• In ELISA, the antibody may be suitable for direct antigen detection or capture; titration is recommended to optimize signal-to-noise.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. For MGMT-related targets in DNA repair and drug resistance research, we provide reliable supply solutions, including custom sourcing for rare targets. Whether you need a single reagent or a complete kit backbone, CamelBio bridges your concept-to-clinic journey with efficient one-stop access.