Anti-MGMT Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16455

Core Product Specifications and Parameters

Biological Background: MGMT Function and Localization

• MGMT, also known as O-6-methylguanine-DNA methyltransferase or O-6-methylguanine-DNA-alkyltransferase, is a DNA repair enzyme.
• It is involved in cellular defense against alkylating agents by repairing O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) lesions in DNA.
• MGMT functions via a suicide mechanism: it stoichiometrically transfers the alkyl group from the DNA lesion to a cysteine residue in its active site, resulting in irreversible inactivation of the enzyme.
• The protein is localized to the nucleus, where it directly binds to DNA and performs its repair function.
• MGMT is a metal-binding (zinc) phosphoprotein, and its activity is linked to resistance to alkylating chemotherapeutic agents in various cancers.
• Keywords associated with MGMT include DNA damage, DNA repair, DNA-binding, methyltransferase, nucleus, phosphoprotein, and zinc.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is approximately 22 kDa; consider using appropriate gel percentage and transfer conditions.
• For immunohistochemistry (IHC-P), antigen retrieval methods may be necessary; validate the antibody in relevant human tissue samples.
• In immunofluorescence (IF/ICC), nuclear localization should be expected; co-staining with a nuclear marker is recommended for confirmation.
• For ELISA, the antibody may be used as a capture or detection reagent; optimal dilutions should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted sourcing partner, we offer validated antibodies like this anti-MGMT monoclonal antibody, along with antibody pairs, bulk ancillary reagents, and rare target sourcing support for DNA repair and oncology research. Streamline your diagnostic development with CamelBio's comprehensive supply chain and expert technical guidance.