Anti-MGMT Rabbit Monoclonal Antibody for WB, ELISA - P16455

Core Product Specifications and Parameters

Biological Background: MGMT Function and Localization

• The official protein name is Methylated-DNA--protein-cysteine methyltransferase, commonly known as O-6-methylguanine-DNA methyltransferase (MGMT). It is also referred to as O-6-methylguanine-DNA-alkyltransferase.
• MGMT is a key DNA repair enzyme that provides cellular defense against the mutagenic and cytotoxic effects of alkylating agents. It specifically removes methyl groups from O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA.
• The repair mechanism is a stoichiometric transfer of the methyl group from the damaged nucleobase to an active-site cysteine residue in the enzyme. This is a suicide reaction, as the enzyme becomes irreversibly inactivated after a single turnover.
• MGMT is predominantly localized in the nucleus, where it encounters genomic DNA lesions.
• The protein binds one zinc ion per subunit, and zinc coordination is likely important for its structural integrity.
• MGMT is regulated by phosphorylation, as indicated by its classification as a phosphoprotein.
• Keywords associated with MGMT include DNA damage, DNA repair, methyltransferase, transferase, metal-binding, and reference proteome, highlighting its established role in maintaining genomic stability.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated against a peptide antigen spanning amino acids 108–207 of human MGMT. It is expected to detect a band at ~22 kDa in Western blot under reducing conditions.
• For Western blotting, consider including a positive control such as human cell lysates known to express MGMT (e.g., HeLa, HEK293). A loading control should be run in parallel.
• For ELISA applications, the antibody can be used as a capture or detection reagent. Indirect or sandwich ELISA formats may be optimized; ensure the coating antigen presents the epitope appropriately.
• Since MGMT expression can vary among human tissues and cell lines, validate the antibody in your experimental system to confirm reactivity and specificity.
• The antibody is validated for human reactivity; cross-reactivity with other species has not been tested, so performance in non-human models cannot be guaranteed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-MGMT clone. With expertise in DNA damage and repair targets, we offer a comprehensive portfolio of antibody pairs, optimized recombinant proteins, and ancillary reagents to accelerate your assay development. Whether you need established catalog products or custom sourcing for rare targets, CamelBio serves as your single point of contact for reliable, scalable solutions.