Anti-MGLL Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q99685

Core Product Specifications and Parameters

Biological Background: MGLL Function and Localization

• MGLL (Monoglyceride lipase), also known as MAGL, HU-K5, lysophospholipase homolog, MGL, and HUK5, is a serine esterase enzyme encoded by the MGLL gene.
• It catalyzes the hydrolysis of monoacylglycerides into free fatty acids and glycerol. Related references: PMID:19029917, PMID:20079333, PMID:21049984
• MGLL hydrolyzes the endocannabinoid 2-arachidonoylglycerol (2-AG), thereby regulating endocannabinoid signaling, nociperception, and pain perception. Related references: PMID:19029917, PMID:20079333, PMID:21049984
• The enzyme regulates levels of fatty acid signaling molecules that promote cancer cell migration, invasion, and tumor growth. Related references: PMID:20079333
• Subcellular localization includes cytoplasm, cytosol, and membrane.
• It is expressed in adipose tissue, lung, liver, kidney, brain, and heart.
• Post-translational modifications include phosphorylation and nitration, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 11-313, encompassing the catalytic domain; consider validating for detection of both full-length (predicted ~33 kDa) and potential isoforms in relevant samples.
• For western blotting (WB), expect a primary band near 33 kDa; optimize antibody dilution and blocking conditions using lysates from tissues with known MGLL expression (e.g., brain, adipose).
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary; test in formalin-fixed paraffin-embedded tissues such as brain or liver sections.
• For ELISA, this antibody can be used as a detection or capture reagent; pairing with a matched antibody pair is recommended for sandwich assays.
• Cross-reactivity with mouse and rat orthologs has been reported; validate specificity in your model system if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD manufacturers, offering a comprehensive range of raw materials and technical support from concept to clinic. For MGLL-related research and diagnostic development, we can supply validated antibody pairs, bulk polyclonal or monoclonal anti-MGLL antibodies, and ancillary reagents. Our sourcing network is adept at procuring rare targets and custom immunogens, ensuring you have single-point access to the critical materials needed for lipid metabolism, endocannabinoid signaling, and oncology assays.