Anti-MGEA5/OGA Monoclonal Antibody for WB, IHC-P, ELISA - O60502

Core Product Specifications and Parameters

Biological Background: MGEA5/OGA Function and Localization

• OGA is a bifunctional O-GlcNAcase that specifically cleaves GlcNAc but not GalNAc from O-glycosylated proteins, regulating dynamic O-GlcNAc modifications on diverse substrates.
  Related references: PMID:11148210, PMID:11788610, PMID:20673219, PMID:28939839, PMID:37962578
• The gene undergoes alternative splicing to produce isoforms with differing enzymatic activities; isoform 1 exhibits approximately six-fold lower specific activity compared to the full-length isoform.
• Localizes to both the nucleus and cytoplasm, consistent with its role in processing nuclear and cytoplasmic O-GlcNAcylated proteins.
• Ubiquitously expressed, with highest levels detected in brain, placenta, and pancreas.
• Annotated as a phosphoprotein, glycosidase, and hydrolase; also implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 433-633 of human MGEA5/OGA (NP_036347.1); consider validating specificity in your relevant sample system.
• For Western blot (WB), a band at approximately 103 kDa is expected; include appropriate positive controls such as brain or placenta lysates.
• For IHC-P and ELISA, optimize antibody dilution and assay conditions according to standard protocols; note the antibody’s cross-reactivity with mouse and rat may facilitate translational studies.
• Always validate under your specific experimental conditions to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. This anti-MGEA5/OGA monoclonal antibody is available through our platform, supporting O-GlcNAcase research and the development of diagnostic assays. From concept to clinic, CamelBio ensures reliable access to critical raw materials for your diagnostic workflows.