Anti-MGAT3 Polyclonal Antibody for WB and ELISA - Q09327

Core Product Specifications and Parameters

Biological Background: MGAT3 Function and Localization

• MGAT3 encodes beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (also known as GNT-III or N-acetylglucosaminyltransferase III), a key glycosyltransferase that adds a bisecting N-acetylglucosamine (GlcNAc) to the beta-linked mannose of the trimannosyl core of N-linked glycans, thereby modifying glycoprotein oligosaccharide structure and function.
• The addition of this bisecting GlcNAc residue suppresses further processing and elongation of N-glycans, precluding the formation of beta-1,6 GlcNAc branching catalyzed by MGAT5, as MGAT5 cannot use the bisected oligosaccharide as a substrate (PubMed:19403558). Related references: PMID:19403558
• MGAT3-catalyzed bisecting GlcNAc modifications impact cell adhesion and migration: addition to CDH1/E-cadherin modulates its cell membrane localization (PubMed:19403558), and inhibition of NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc- formation by MGAT3 regulates sialylation and cell migration (PubMed:26801611). Related references: PMID:19403558 PMID:26801611
• In the brain, MGAT3 adds bisecting GlcNAc to BACE1, blocking its lysosomal targeting under oxidative stress and increasing its localization to early endosomes, which enhances APP cleavage and amyloid beta generation.
• MGAT3 modifies IgG Fc N-glycans by adding bisecting GlcNAc to complex-type N-linked glycans, affecting antibody-dependent cellular cytotoxicity (ADCC) and potentially therapeutic antibody functions.
• The enzyme is localized to the Golgi apparatus membrane, specifically the Golgi stack membrane, consistent with its role in late-stage N-glycan processing.
• Alternative names include GNT3, GNT-III, and N-acetylglucosaminyltransferase III; the protein is predicted to contain a transmembrane helix and a signal-anchor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 364–533, a region accessible for antibody generation; consider using this antibody in Western blot under reducing conditions, where a major band around 61kDa may be observed.
• For ELISA, optimize coating conditions and validate in the intended sample matrix, as glycosylation patterns and target abundance may vary between tissues or cell lines.
• Given the reported cross-reactivity with mouse and rat, validate target specificity in these species by sequence alignment or additional controls, especially if used in non-human samples.
• Since MGAT3 is a Golgi-resident protein, consider appropriate sample lysis and fractionation protocols to enrich membrane compartments for more robust detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials for diagnostic developers and research laboratories. With expertise in glycobiology and enzyme targets like MGAT3, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials—ensuring consistent supply from concept to clinic.