Anti-MGAT3 Monoclonal Antibody for WB, ELISA - Q09327

Core Product Specifications and Parameters

Biological Background: MGAT3 Function and Localization

• MGAT3, also known as GNT-III (N-acetylglucosaminyltransferase III), is a glycosyltransferase that catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta-1,4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains, forming bisecting GlcNAc.
• The bisecting GlcNAc moiety alters N-glycan conformation and suppresses further processing, including the formation of beta-1,6 GlcNAc branching by MGAT5, which cannot utilize bisected substrates. Related references: PMID:19403558
• MGAT3-mediated bisecting GlcNAc addition to CDH1/E-cadherin modulates its cell membrane localization. Related references: PMID:19403558
• It inhibits the formation of NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc- structures, thereby regulating sialylation levels and influencing cell migration. Related references: PMID:26801611
• In the brain, MGAT3 adds bisecting GlcNAc to BACE1, which blocks its lysosomal targeting under oxidative stress, increasing early endosome localization and APP cleavage (By similarity).
• MGAT3 modifies complex-type N-linked glycans on IgG Fc regions, converting various core glycoforms; galactosylation on the alpha(1->6) Man branch is permissive.
• Located in the Golgi stack membrane, MGAT3 is a single-pass type II membrane protein with a signal-anchor topology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 381-480 of human MGAT3. Verify antibody recognition of the full-length endogenous protein under your experimental conditions, as peptide antibodies may not always detect native conformations.
• For Western blotting, consider using reducing conditions and a molecular weight marker around 61 kDa. Validate in the relevant sample system, as expression levels and post-translational modifications can vary.
• In ELISA applications, optimize coating antigen and antibody concentrations to achieve the desired sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies for glycobiology targets like MGAT3. We offer reliable access to bulk ancillary reagents and can assist in sourcing rare-target monoclonal antibodies to accelerate your assay development and biomarker research from concept to clinic.