Anti-MGAT2 Rabbit Polyclonal Antibody for WB and ELISA - Q10469

Core Product Specifications and Parameters

Biological Background: MGAT2 Function and Localization

• MGAT2, also known as GlcNAc-T II or GNT-II, is a Golgi-resident enzyme that catalyzes the addition of N-acetylglucosamine (GlcNAc) to the core mannose of N-linked glycans, forming the second branch of complex glycoproteins.
• It is essential for proper protein N-glycosylation, impacting glycoprotein folding, stability, and function.
• The protein is localized to the Golgi apparatus membrane, with a type II transmembrane topology (signal-anchor).
• MGAT2 contains disulfide bonds and requires manganese as a cofactor for catalytic activity.
• Mutations in MGAT2 are associated with congenital disorders of glycosylation type IIa (CDG2A), highlighting its clinical significance.
• Alternative names include GNT2, CDG2A, CDGS2, and GLCNACTII.
• The molecular weight of the glycosylated pro-form is approximately 52 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 178–447, covering the catalytic domain; consider epitope mapping for critical applications.
• For Western blot, a band around 52 kDa is expected in human, mouse, and rat lysates; validate with positive controls expressing MGAT2, as glycosylation may cause slight shifts.
• In ELISA, optimal coating conditions and antibody dilution should be empirically determined; the antibody may be used to detect MGAT2 in cell or tissue extracts.
• Cross-reactivity with other species should be tested individually by aligning the immunogen sequence with target orthologs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. This Anti-MGAT2 Rabbit Polyclonal Antibody supports glycobiology and CDG research, enabling robust detection in WB and ELISA. We streamline procurement from discovery to diagnostic development.