Anti-MGAT1 Polyclonal Antibody for WB, ELISA - P26572

Core Product Specifications and Parameters

Biological Background: MGAT1 Function and Localization

• MGAT1, also known as alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase I (GNT-I), is encoded by the MGAT1 gene in humans.
• This enzyme initiates the formation of complex N-linked carbohydrates by catalyzing the transfer of N-acetylglucosamine (GlcNAc) to the core mannose of high-mannose N-glycans.
• MGAT1 is essential for the conversion of high-mannose type N-glycans to hybrid and complex N-glycans, a critical step in glycoprotein processing and maturation.
• It localizes to the Golgi apparatus membrane, specifically in the cis/medial-Golgi, and also shows cytoplasmic perinuclear distribution.
• MGAT1 belongs to the glycosyltransferase 13 family, and its activity requires manganese as a cofactor for catalytic function.
• The protein contains a transmembrane domain and signal-anchor, anchoring it to the membrane, and features disulfide bonds important for structural integrity.
• MGAT1 is included in the human reference proteome and has been identified in multiple high-throughput proteomics studies, underscoring its fundamental biological role.

Experimental Guidance and Technical Tips

• For Western blot applications, the predicted molecular weight is approximately 51 kDa; however, consider potential band shifts due to glycosylation or other post-translational modifications of the target or interacting proteins.
• The immunogen is located within the N-terminal region (amino acids 1-100), which may detect both full-length membrane-anchored and any soluble or processed forms, so validate in your specific sample context.
• When designing ELISA assays, optimize antigen coating and detection conditions, and note that the antibody recognizes human and rat MGAT1, so it may be suitable for cross-species studies with appropriate validation.
• Given the polyclonal nature, the antibody may exhibit batch-to-batch variability; it is recommended to validate each new lot before use in critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer a wide range of validated antibodies, including anti-MGAT1 products, along with optimized monoclonal and polyclonal antibody development services. For your glycobiology and Golgi-related projects, our team can also source rare targets and deliver ancillary reagents in bulk, helping streamline your assay development and scale-up processes.