Anti-MFSD2A/NLS1 Rabbit Monoclonal Antibody for WB, ELISA - Q8NA29

Core Product Specifications and Parameters

Biological Background: MFSD2A/NLS1 Function and Localization

• Encodes a sodium-dependent lysophosphatidylcholine (LPC) symporter that is essential for blood-brain barrier (BBB) formation and function, mediating LPC transport across the endothelium. Related references: PMID:24828040, PMID:32572202, PMID:34135507
• Serves as the major route for brain uptake of docosahexaenoic acid (DHA), an omega-3 fatty acid critical for neurodevelopment, by transporting LPC-DHA rather than unesterified DHA. Related references: PMID:26005868, PMID:34135507
• Transports LPC species with long-chain fatty acids (minimum 14-carbon acyl chain), such as LPC-oleate and LPC-palmitate, but does not transport free fatty acids.
• In placenta, acts as a receptor for the endogenous retroviral envelope protein syncytin-2 (ERVFRD-1), a function required for trophoblast cell fusion during syncytiotrophoblast formation. Related references: PMID:18988732, PMID:23177091
• Also capable of transporting tunicamycin, an inhibitor of asparagine-linked glycosylation, highlighting a secondary substrate specificity. Related references: PMID:21677192
• Localizes primarily to the cell membrane and is also detected at the endoplasmic reticulum membrane, consistent with its roles in transport and receptor activity.
• Belongs to the major facilitator superfamily (MFS) of transporters; undergoes alternative splicing and is classified under keywords including lipid transport, symport, and primary microcephaly.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant human protein; therefore, consider using recombinant MFSD2A or peptide fragments as positive controls in ELISA and WB.
• For Western blot, prepare whole-cell or membrane-enriched lysates from human, mouse, or rat brain microvessel endothelial cells or placental tissue where MFSD2A is expressed.
• Due to the predicted molecular weight of approximately 60 kDa, include appropriate molecular weight markers and optimize transfer conditions for proteins of this size.
• As with any antibody, titrate the working concentration for your specific sample type and application; cross-reactivity with rat and mouse has been reported but validate in your system.
• Note that MFSD2A may undergo glycosylation and other modifications; observed band sizes may differ slightly from the theoretical 60 kDa.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with one-stop access to high-quality IVD raw materials, technical services, and consulting from concept to clinic. This MFSD2A monoclonal antibody supports research into blood-brain barrier physiology, neurodevelopmental disorders, and placental biology. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—enabling you to advance your projects efficiently.