Anti-MFSD2A Polyclonal Antibody for WB, ELISA - Q8NA29

Core Product Specifications and Parameters

Biological Background: MFSD2A Function and Localization

• MFSD2A is a sodium-dependent lysophosphatidylcholine (LPC) symporter essential for blood-brain barrier (BBB) formation and function. Related references: PMID:24828040 PMID:32572202 PMID:34135507
• It transports LPC into the brain, which constitutes the major pathway for docosahexaenoic acid (DHA) delivery critical for normal brain growth and cognitive function. Related references: PMID:26005868 PMID:34135507
• The protein belongs to the major facilitator superfamily (MFS) and is specifically expressed in endothelial cells of the BBB.
• Subcellularly, MFSD2A localizes to the cell membrane and endoplasmic reticulum membrane.
• In the placenta, it acts as a receptor for syncytin-2 and is required for trophoblast fusion. Related references: PMID:18988732 PMID:23177091
• MFSD2A also functions as a transporter for tunicamycin, an inhibitor of N-linked glycosylation. Related references: PMID:21677192
• It is a glycoprotein with disulfide bonds, contains multiple transmembrane helices, and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein corresponding to amino acids 464-543 (C-terminal region) of human MFSD2A, and may cross-react with mouse and rat orthologs.
• For Western blot, consider using whole cell lysates from human, mouse, or rat tissues known to express MFSD2A (e.g., brain, placenta). The predicted molecular weight is approximately 60 kDa; however, glycosylation may cause a shift in migration.
• For ELISA, titrate the antibody against the immunogen or native protein to determine the optimal working concentration. Validate specificity with appropriate controls.
• Always include a positive control (e.g., human brain microvascular endothelial cells or placental tissue) and a negative control to confirm detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers, laboratories, and research institutes. This MFSD2A polyclonal antibody supports studies in lipid transport, blood-brain barrier biology, and placental development. Our portfolio also includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets—positioning CamelBio as a comprehensive partner from discovery to clinical application.