Anti-MFI2 (Melanotransferrin) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08582

Core Product Specifications and Parameters

Biological Background: MFI2 Function and Localization

• Melanotransferrin (MFI2), also known as melanoma-associated antigen p97 or CD228, is a cell-surface glycoprotein belonging to the transferrin family, capable of binding one iron atom per subunit.
• It mediates cellular iron uptake through receptor-mediated endocytosis, with the protein internalizing bound iron and then recycling back to the cell membrane; it may also transport zinc.
• The protein is attached to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor; alternative splicing generates a secreted isoform lacking the anchor.
• Subcellularly, it localizes primarily to the cell membrane, consistent with its function in metal ion transport across the plasma membrane.
• Expression is prominent in human melanomas and certain fetal tissues, with lower levels detected in liver, epithelium, umbilical cord, placenta, and sweat gland ducts.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, as indicated by proteomics and structural studies.
• Functional keywords highlight its involvement in ion transport, iron transport, and metal-binding, and its classification as a lipoprotein and GPI-anchored membrane protein.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of melanotransferrin is approximately 80 kDa; expect a specific band near this size under reducing conditions, and validate detection using relevant human, mouse, or rat lysates.
• In immunofluorescence (IF/ICC), since the target localizes to the cell membrane, consider live-cell staining without permeabilization to detect surface receptor, or employ mild permeabilization for total cellular staining; optimize fixation to preserve epitope integrity.
• The immunogen corresponds to the extracellular region (amino acids 20–200), making the antibody suitable for recognizing the native receptor on live cells.
• For ELISA applications, the antibody can be evaluated as a capture or detection reagent using recombinant protein or native standards; titration and pairing experiments are recommended.
• While cross-reactivity with mouse and rat is reported, verify endogenous expression in your specific model system, as levels may vary across tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, offering this anti-MFI2 polyclonal antibody alongside validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With deep knowledge in iron transport and melanoma research targets, we support diagnostic manufacturers from concept to clinic by streamlining procurement and delivering technical consulting services. Whether you require rare target raw materials or reliable detection components, CamelBio bridges the gap between research and diagnostic development.