Anti-MFI2 (Melanotransferrin) Rabbit Monoclonal Antibody for WB, FC, ELISA - P08582

Core Product Specifications and Parameters

Biological Background: Melanotransferrin Function and Localization

• Melanotransferrin (MFI2, CD228, p97) is a cell surface glycoprotein that mediates cellular iron uptake by binding a single iron atom per subunit and potentially zinc.
• The protein undergoes internalization and subsequent recycling back to the plasma membrane, consistent with a role in iron transport.
• Tissue expression is prominent in human melanomas and certain fetal tissues, alongside liver, epithelium, umbilical cord, placenta, and sweat gland ducts.
• As a GPI-anchored membrane protein, MFI2 features disulfide bonds, signal peptide, and multiple repeat domains, contributing to its structural stability.
• Alternative splicing generates isoforms, and post-translational modifications include phosphorylation according to proteomics data.
• Its molecular weight is approximately 80 kDa, although a 32 kDa fragment may exist as indicated by product specifications.
• Keywords highlight its involvement in metal ion binding (iron, zinc), transport, and cell membrane localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–708 of human MFI2, covering the majority of the extracellular domain; this broad coverage may facilitate detection of both full-length and truncated forms.
• For Western blotting under reducing conditions, anticipate bands near 80 kDa (full-length) and possibly a 32 kDa fragment; optimize sample preparation to preserve protein integrity.
• Flow cytometry can leverage the antibody's reactivity with the native cell surface protein, particularly in melanoma or other MFI2-expressing cells; include appropriate isotype controls.
• ELISA applications may use recombinant protein or cell lysates; pairing with a matched detection antibody may improve sensitivity.
• Cross-reactivity with rat is documented, but validation in the specific experimental system is recommended before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides an integrated sourcing solution for IVD raw materials, supporting manufacturers from concept to clinic. For targets like melanotransferrin in iron metabolism and melanoma research, we supply validated monoclonal/polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to accelerate assay development. Our comprehensive procurement platform also specializes in rare target raw materials, ensuring reliable access to the critical components your diagnostic workflows demand.