Anti-MFG-E8 Monoclonal Antibody for WB, IF/ICC, ELISA - Q08431

Core Product Specifications and Parameters

Biological Background: MFG-E8 Function and Localization

• Lactadherin (MFG-E8) is a 43 kDa glycoprotein encoded by the MFGE8 gene; alternative splicing may produce multiple isoforms, yet the major form consistently contains EGF-like and discoidin-like F5/8 type C domains.
• It promotes intestinal epithelial homeostasis and mucosal healing, while also supporting VEGF-dependent neovascularization (angiogenesis).
• MFG-E8 functions as a key bridging molecule for phagocytic clearance of apoptotic cells: it binds phosphatidylserine on dying cells and engages integrins αvβ3 and αvβ5 on phagocytes.
• The protein can directly attach to phosphatidylserine-enriched membranes in a receptor-independent manner and interacts with the zona pellucida, suggesting a role in sperm-egg binding.
• It is a main constituent of aortic medial amyloid, linking it to pathological vascular deposits.
• Subcellularly, MFG-E8 is found on the cell membrane, secreted into the extracellular space, and inside cytoplasmic/secretory vesicles as well as the acrosome membrane.
• Tissue specificity includes mammary epithelial cells, aortic media, and overexpression in several carcinomas; it is also present in milk fat globules.
• Post-translational modifications feature disulfide bonds, glycosylation, and phosphorylation; the protein is classified under keywords such as amyloid, angiogenesis, cell adhesion, fertilization, and secreted reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal domain (amino acids 231–387) of human MFG-E8, which is expected to generate antibodies recognizing the full-length ~43 kDa protein in western blotting; consider loading 20–30 µg of reduced lysate.
• Due to cross-reactivity with mouse and rat, the antibody is suitable for multi-species studies; always validate expression levels in the chosen sample system.
• For IF/ICC, permeabilization is recommended because MFG-E8 localizes to membranes and vesicles; optimize fixation and antibody dilution to minimize background.
• In ELISA applications, the antibody can serve as a capture or detection reagent; pairing with a second antibody that recognizes a distinct epitope may improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. The MFG-E8 Rabbit mAb fits apoptosis, angiogenesis, and cancer biomarker workflows, and our team can assist in scaling custom MFG-E8 antibody lots or related reagents to meet your clinical or translational development goals.