Anti-MFF Rabbit Polyclonal Antibody (WB, IHC-P, ELISA) - Q9GZY8

Core Product Specifications and Parameters

Biological Background: MFF Function and Localization

• MFF (Mitochondrial fission factor) is a crucial regulator of mitochondrial and peroxisomal fission [PMID:18353969, PMID:23530241, PMID:24196833]. Related references: PMID:18353969, PMID:23530241, PMID:24196833
• It facilitates the recruitment and association of dynamin-related protein 1 (DNM1L) to the mitochondrial outer membrane, a key step in fission [PMID:23530241]. Related references: PMID:23530241
• MFF may be involved in the regulation of synaptic vesicle membrane dynamics by recruiting DNM1L to clathrin-containing vesicles (By similarity).
• The protein localizes to the mitochondrion outer membrane, peroxisomes, and cytoplasmic vesicles including secretory and synaptic vesicles.
• MFF is highly expressed in heart, kidney, liver, brain, muscle, and stomach.
• Post-translational modifications include phosphorylation, and the protein contains a transmembrane domain with alternative splice isoforms.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using reduced and denatured lysates from human, mouse, or rat tissues with high MFF expression (e.g., heart, brain, or liver). A band around 38 kDa is expected.
• For immunohistochemistry (IHC-P), appropriate antigen retrieval methods and endogenous peroxidase blocking are recommended. Validate with appropriate positive and negative tissue controls.
• For ELISA, the peptide immunogen (aa 1-100) can be used as a standard or coating antigen to establish assay sensitivity. Cross-reactivity with other species may require sequence alignment verification.
• As a polyclonal antibody, lot-to-lot consistency should be monitored, and we recommend validating the antibody in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutions a streamlined pathway to IVD raw materials, with extensive coverage in mitochondrial biology and organelle dynamics. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, including those for rare targets like MFF. Our sourcing network enables consistent access to high-quality raw materials to accelerate your development from concept to clinic.