Anti-MFAP5 Rabbit Polyclonal Antibody for WB, ELISA - Q13361

Core Product Specifications and Parameters

Biological Background: MFAP5 Function and Localization

• MFAP5 (Microfibrillar-associated protein 5), also designated MAGP-2 (Microfibril-associated glycoprotein 2) or MP25, is an extracellular matrix glycoprotein encoded by the MFAP5 gene.
• It is a key component of elastin-associated microfibrils, contributing to the structural integrity of elastic fibers. Related references: PMID:8557636
• May play a role in hematopoiesis, potentially influencing blood cell development (By similarity).
• In the cardiovascular system, MFAP5 is implicated in regulating growth factors and cell signaling to maintain large vessel integrity (By similarity).
• The protein is secreted into the extracellular space and localizes to the extracellular matrix.
• MFAP5 undergoes post-translational modifications including disulfide bond formation and glycosylation, which may be critical for its function.
• Alternative splicing generates multiple isoforms, and mutations in MFAP5 are associated with disease variants such as aortic aneurysm.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 73–173 of human MFAP5; verify sequence conservation if using samples from non-listed species.
• For Western blotting, expect a band near the predicted molecular weight of 20 kDa; optimize gel percentage and transfer conditions for low-molecular-weight proteins.
• In ELISA, consider using a direct or sandwich format and validate with recombinant protein or appropriate positive controls.
• Cross-reactivity with mouse and rat suggests potential utility in rodent models; confirm performance in the specific tissue or cell system of interest.
• As a secreted extracellular matrix protein, sample preparation may benefit from enrichment of ECM fractions or conditioned medium.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-MFAP5 polyclonal antibody supports investigations into extracellular matrix biology, cardiovascular function, and related disease mechanisms. Whether you need optimized antibodies, recombinant proteins, or sourcing of rare targets, CamelBio bridges the gap between concept and clinical application with scalable supply solutions.